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anti ace2  (Sino Biological)


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    Sino Biological anti ace2
    Anti Ace2, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+ace2/ACE2+Antibody%2C+Rabbit+PAb%2C+Antigen+Affinity+Purified/pm41957014-206-4-5
    Average 94 stars, based on 22 article reviews
    anti ace2 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Staining:

    Article Title: DAZAP2 functions as a pan-coronavirus restriction factor by inhibiting viral entry and genomic replication.
    Article Snippet: After two additional washes, cells were subjected to flow cytometry analysis (Thermo, Attune NxT) and data processing (FlowJo v10.0.7). .. For surface staining of entry-related host factors and analyzed by flow cytometry, cells were collected with TrypLE (Thermo Fisher #12605010) and incubated with the rabbit anti-ACE2 (Sino Biological #10108-RP01, 1:500), mouse anti-AXL (R&D #MAB154SP, 1 μg/mL), mouse anti-DC-SIGN (Biolegend #330102, 1 μg/mL), mouse anti-TIM-1 (Biolegend #354002, 1 μg/mL), mouse anti-SIGLEC1 (Abcam #ab199401, 1 μg/mL), or mouse anti-heparan sulfate (10E4) (USBiological #H1890, 1 μg/mL) primary antibody at 4°C for 30 min. After washing, cells were stained with goat anti-rabbit IgG (H + L) conjugated with Alexa Fluor 647 (Thermo Fisher #A21245, 2 μg/mL) or goat anti-mouse IgG (H + L) conjugated with Alexa Fluor 647 (Thermo fisher #A21235, 2 μg/mL) for 30 min at 4°C and subjected to flow cytometry analysis. ..

    Article Title: DAZAP2 functions as a pan-coronavirus restriction factor by inhibiting viral entry and genomic replication
    Article Snippet: After two additional washes, cells were subjected to flow cytometry analysis (Thermo, AttuneTM NxT) and data processing (FlowJo v10.0.7). .. For surface staining of entry-related host factors and analyzed by flow cytometry, cells were collected with TrypLE (Thermo fisher #12605010) and incubated with the rabbit anti-ACE2 (Sino Biological #10108-RP01, 1:500), mouse anti-AXL (R&D #MAB154-SP, 1 μg/ml), mouse anti-DC-SIGN (Biolegend #330102, 1 μg/ml), mouse anti-TIM-1 (Biolegend #354002, 1 μg/ml), mouse anti-SIGLEC1 (Abcam #ab199401, 1 μg/ml), or mouse anti-heparan sulfate (10E4) (USBiological #H1890, 1 μg/ml) primary antibody at 4 °C for 30 min. After washing, cells were stained with goat anti-rabbit IgG (H + L) conjugated with Alexa Fluor 647 (Thermo fisher #A21245, 2 μg/ml) or goat anti-mouse IgG (H + L) conjugated with Alexa Fluor 647 (Thermo fisher #A21235, 2 μg/ml) for 30 min at 4 °C and subjected to flow cytometry analysis. ..

    Article Title: DAZAP2 functions as a pan-coronavirus restriction factor by inhibiting viral entry and genomic replication
    Article Snippet: After two additional washes, cells were subjected to flow cytometry analysis (Thermo, Attune NxT) and data processing (FlowJo v10.0.7). .. For surface staining of entry-related host factors and analyzed by flow cytometry, cells were collected with TrypLE (Thermo Fisher #12605010) and incubated with the rabbit anti-ACE2 (Sino Biological #10108-RP01, 1:500), mouse anti-AXL (R&D #MAB154-SP, 1 μg/mL), mouse anti-DC-SIGN (Biolegend #330102, 1 μg/mL), mouse anti-TIM-1 (Biolegend #354002, 1 μg/mL), mouse anti-SIGLEC1 (Abcam #ab199401, 1 μg/mL), or mouse anti-heparan sulfate (10E4) (USBiological #H1890, 1 μg/mL) primary antibody at 4°C for 30 min. After washing, cells were stained with goat anti-rabbit IgG (H + L) conjugated with Alexa Fluor 647 (Thermo Fisher #A21245, 2 μg/mL) or goat anti-mouse IgG (H + L) conjugated with Alexa Fluor 647 (Thermo fisher #A21235, 2 μg/mL) for 30 min at 4°C and subjected to flow cytometry analysis. ..

    Flow Cytometry:

    Article Title: DAZAP2 functions as a pan-coronavirus restriction factor by inhibiting viral entry and genomic replication.
    Article Snippet: After two additional washes, cells were subjected to flow cytometry analysis (Thermo, Attune NxT) and data processing (FlowJo v10.0.7). .. For surface staining of entry-related host factors and analyzed by flow cytometry, cells were collected with TrypLE (Thermo Fisher #12605010) and incubated with the rabbit anti-ACE2 (Sino Biological #10108-RP01, 1:500), mouse anti-AXL (R&D #MAB154SP, 1 μg/mL), mouse anti-DC-SIGN (Biolegend #330102, 1 μg/mL), mouse anti-TIM-1 (Biolegend #354002, 1 μg/mL), mouse anti-SIGLEC1 (Abcam #ab199401, 1 μg/mL), or mouse anti-heparan sulfate (10E4) (USBiological #H1890, 1 μg/mL) primary antibody at 4°C for 30 min. After washing, cells were stained with goat anti-rabbit IgG (H + L) conjugated with Alexa Fluor 647 (Thermo Fisher #A21245, 2 μg/mL) or goat anti-mouse IgG (H + L) conjugated with Alexa Fluor 647 (Thermo fisher #A21235, 2 μg/mL) for 30 min at 4°C and subjected to flow cytometry analysis. ..

    Article Title: DAZAP2 functions as a pan-coronavirus restriction factor by inhibiting viral entry and genomic replication
    Article Snippet: After two additional washes, cells were subjected to flow cytometry analysis (Thermo, AttuneTM NxT) and data processing (FlowJo v10.0.7). .. For surface staining of entry-related host factors and analyzed by flow cytometry, cells were collected with TrypLE (Thermo fisher #12605010) and incubated with the rabbit anti-ACE2 (Sino Biological #10108-RP01, 1:500), mouse anti-AXL (R&D #MAB154-SP, 1 μg/ml), mouse anti-DC-SIGN (Biolegend #330102, 1 μg/ml), mouse anti-TIM-1 (Biolegend #354002, 1 μg/ml), mouse anti-SIGLEC1 (Abcam #ab199401, 1 μg/ml), or mouse anti-heparan sulfate (10E4) (USBiological #H1890, 1 μg/ml) primary antibody at 4 °C for 30 min. After washing, cells were stained with goat anti-rabbit IgG (H + L) conjugated with Alexa Fluor 647 (Thermo fisher #A21245, 2 μg/ml) or goat anti-mouse IgG (H + L) conjugated with Alexa Fluor 647 (Thermo fisher #A21235, 2 μg/ml) for 30 min at 4 °C and subjected to flow cytometry analysis. ..

    Article Title: DAZAP2 functions as a pan-coronavirus restriction factor by inhibiting viral entry and genomic replication
    Article Snippet: After two additional washes, cells were subjected to flow cytometry analysis (Thermo, Attune NxT) and data processing (FlowJo v10.0.7). .. For surface staining of entry-related host factors and analyzed by flow cytometry, cells were collected with TrypLE (Thermo Fisher #12605010) and incubated with the rabbit anti-ACE2 (Sino Biological #10108-RP01, 1:500), mouse anti-AXL (R&D #MAB154-SP, 1 μg/mL), mouse anti-DC-SIGN (Biolegend #330102, 1 μg/mL), mouse anti-TIM-1 (Biolegend #354002, 1 μg/mL), mouse anti-SIGLEC1 (Abcam #ab199401, 1 μg/mL), or mouse anti-heparan sulfate (10E4) (USBiological #H1890, 1 μg/mL) primary antibody at 4°C for 30 min. After washing, cells were stained with goat anti-rabbit IgG (H + L) conjugated with Alexa Fluor 647 (Thermo Fisher #A21245, 2 μg/mL) or goat anti-mouse IgG (H + L) conjugated with Alexa Fluor 647 (Thermo fisher #A21235, 2 μg/mL) for 30 min at 4°C and subjected to flow cytometry analysis. ..

    Incubation:

    Article Title: DAZAP2 functions as a pan-coronavirus restriction factor by inhibiting viral entry and genomic replication.
    Article Snippet: After two additional washes, cells were subjected to flow cytometry analysis (Thermo, Attune NxT) and data processing (FlowJo v10.0.7). .. For surface staining of entry-related host factors and analyzed by flow cytometry, cells were collected with TrypLE (Thermo Fisher #12605010) and incubated with the rabbit anti-ACE2 (Sino Biological #10108-RP01, 1:500), mouse anti-AXL (R&D #MAB154SP, 1 μg/mL), mouse anti-DC-SIGN (Biolegend #330102, 1 μg/mL), mouse anti-TIM-1 (Biolegend #354002, 1 μg/mL), mouse anti-SIGLEC1 (Abcam #ab199401, 1 μg/mL), or mouse anti-heparan sulfate (10E4) (USBiological #H1890, 1 μg/mL) primary antibody at 4°C for 30 min. After washing, cells were stained with goat anti-rabbit IgG (H + L) conjugated with Alexa Fluor 647 (Thermo Fisher #A21245, 2 μg/mL) or goat anti-mouse IgG (H + L) conjugated with Alexa Fluor 647 (Thermo fisher #A21235, 2 μg/mL) for 30 min at 4°C and subjected to flow cytometry analysis. ..

    Article Title: DAZAP2 functions as a pan-coronavirus restriction factor by inhibiting viral entry and genomic replication
    Article Snippet: After two additional washes, cells were subjected to flow cytometry analysis (Thermo, AttuneTM NxT) and data processing (FlowJo v10.0.7). .. For surface staining of entry-related host factors and analyzed by flow cytometry, cells were collected with TrypLE (Thermo fisher #12605010) and incubated with the rabbit anti-ACE2 (Sino Biological #10108-RP01, 1:500), mouse anti-AXL (R&D #MAB154-SP, 1 μg/ml), mouse anti-DC-SIGN (Biolegend #330102, 1 μg/ml), mouse anti-TIM-1 (Biolegend #354002, 1 μg/ml), mouse anti-SIGLEC1 (Abcam #ab199401, 1 μg/ml), or mouse anti-heparan sulfate (10E4) (USBiological #H1890, 1 μg/ml) primary antibody at 4 °C for 30 min. After washing, cells were stained with goat anti-rabbit IgG (H + L) conjugated with Alexa Fluor 647 (Thermo fisher #A21245, 2 μg/ml) or goat anti-mouse IgG (H + L) conjugated with Alexa Fluor 647 (Thermo fisher #A21235, 2 μg/ml) for 30 min at 4 °C and subjected to flow cytometry analysis. ..

    Article Title: DAZAP2 functions as a pan-coronavirus restriction factor by inhibiting viral entry and genomic replication
    Article Snippet: After two additional washes, cells were subjected to flow cytometry analysis (Thermo, Attune NxT) and data processing (FlowJo v10.0.7). .. For surface staining of entry-related host factors and analyzed by flow cytometry, cells were collected with TrypLE (Thermo Fisher #12605010) and incubated with the rabbit anti-ACE2 (Sino Biological #10108-RP01, 1:500), mouse anti-AXL (R&D #MAB154-SP, 1 μg/mL), mouse anti-DC-SIGN (Biolegend #330102, 1 μg/mL), mouse anti-TIM-1 (Biolegend #354002, 1 μg/mL), mouse anti-SIGLEC1 (Abcam #ab199401, 1 μg/mL), or mouse anti-heparan sulfate (10E4) (USBiological #H1890, 1 μg/mL) primary antibody at 4°C for 30 min. After washing, cells were stained with goat anti-rabbit IgG (H + L) conjugated with Alexa Fluor 647 (Thermo Fisher #A21245, 2 μg/mL) or goat anti-mouse IgG (H + L) conjugated with Alexa Fluor 647 (Thermo fisher #A21235, 2 μg/mL) for 30 min at 4°C and subjected to flow cytometry analysis. ..

    other:

    Article Title: SARS-CoV-2 infection in brain tumors and the association with alterations in the tumor immune microenvironment.
    Article Snippet: The following antibodies were used in the current study: anti-SARS-CoV-2-NP (40143-MM05, 1:100; Sino Biological, Beijing, china); anti-NRP1 (60067-1-Ig, 1:200; Proteintech, Rsemont, IL, USA); rabbit anti-CD31 (77699S, 1:500; Cell Signaling Technology, Danvers, MA, USA); rabbit anti-ACE2 (10108-T24, 1:200; Sino Biological); rabbit anti-TMPRSS2 (14437-1-AP, 1:200; Proteintech); mouse anti-CD147/BSG (66443-1-Ig, 1:200; Proteintech); anti-CD3 (85061s, 1:300; CST, Danvers, MA, USA); anti-CD4 (ab133616, 1:1000; Abcam, Cambridge, United Kingdom); anti-CD8 (ab93278, 1:200; Abcam); anti-CD8 (66868-1-Ig, 1:200; Proteintech); anti-CD20 (ab9475, 1:1000; Abcam); anti-CD68 (76437s, 1:500; CST); anti-C3 (ET7106-75, 1:100; Huabio, Fort Worth, TX, USA); anti-C1q (HA721439, 1:100; Huabio); anti-IgG (ZA-0448; ZSGB-BIO, Beijing, China); anti-IgM (ZA-0450; ZSGB-BIO); anti-TTF1 (66034-1-Ig, 1:200; Proteintech); rabbit anti-GFAP (16825-1-AP, 1:200; Proteintech); anti-MUC1 (HA601142, 1:200; Huabio); anti-panCK (HA601138, 1:200; Huabio); anti-PD-1 (18106-1-AP, 1:200; Proteintech); antiIFN-γ (AB231036, 1:200; Abcam); and anti-FOXP3 (22228-1- AP, 1:200; Proteintech).

    Article Title: Rab27-dependent egress of SARS-CoV-2 via secretory amphisomes
    Article Snippet: Rabbit anti-ACE2 (Sino Biological, #80031-RP02), mouse beta-tubulin IV (BioGenex, #MU178-UC), mouse anti-CC10 (Origene, AM26360PU-N), mouse anti-CD63 (DSHB Hybridoma Product H5C6, deposited to the DSHB by August, J.T.

    Article Title: Optical nanoscopy reveals SARS-CoV-2-induced remodeling of human airway cells
    Article Snippet: Primary antibodies used in this study were rabbit anti-ACE2 (Sino Biological, #80031-RP02), mouse beta-tubulin IV (BioGenex, #MU178-UC), rabbit anti-Calnexin (Abcam, ab22595), mouse anti-CC10 (Origene, AM26360PU-N), Mouse-anti-CD63 (DSHB Hybridoma Product H5C6, deposited to the DSHB by August, J.T.

    Western Blot:

    Article Title: Sensing of cytoplasmic chromatin by cGAS activates innate immune response in SARS-CoV-2 infection
    Article Snippet: .. The antibodies used for immunoblotting were: rabbit-anti-cGAS (D1D3G, #15102 S, Cell Signaling Technology, 1:500), rabbit-anti-STING (D2P2F, #13647 S, Cell Signaling Technology, 1:500), rabbit-anti-phospho-STING (Ser366) (D7C3S, #19781 S, Cell Signaling Technology, 1:500), rabbit-anti-IRF3 (ab76409, Abcam, 1:1000), rabbi-anti-phospho-IRF3 (ab76493, Abcam, 1:1000), rabbit-anti-GAPDH (BE0024, EASYBIO, 1:2000), mouse-anti-β-Tubulin (CW0098, CWBIO, 1:2000), rabbit-anti-SARS-CoV-2 spike protein (40589-T62, Sino Biological, 1:1000), rabbit-anti-SARS-CoV-2 nucleocapsid protein (NP) (40143-R019, Sino Biological, 1:1000), rabbit-anti-Sendai virus (PD029C1, MBL, 1:1000), rabbit-anti-ACE2 (10108-T60, Sino Biological, 1:1000), mouse-anti-MAVS (sc166583, Santa Cruz, 1:500), goat-anti-mouse IgG-HRP secondary antibody (115035003, Jackson ImmunoResearch), mouse-anti-Lamin A/C (MABT538, Sigma-Aldrich, 1:500), rabbit-anti-Lamin B1 (ab16048, Abcam, 1:500), goat-anti-rabbit IgG-HRP secondary antibody (111035003, Jackson ImmunoResearch). ..

    Article Title: LL-37 Inhibits TMPRSS2-Mediated S2' Site Cleavage and SARS-CoV-2 Infection but Not Omicron Variants.
    Article Snippet: Restore PLUS Western Blot Stripping Buffer (46430, ThermoFisher, USA) was used to strip the antibodies before re- incubation of membranes. .. Primary antibodies used for western blot analysis included rabbit anti- SARS- CoV- 2 (2019- nCoV) spike S1 (Sino Biological, 40591- T62), rabbit anti- SARS- CoV- 2 (2019- nCoV) spike S2 (Sino Biological, 40590- T62), rabbit anti- Cathelicidin/ CLP (Abcam, ab207758), rabbit anti- ACE2 (Sino Biological, 10108- R003), rabbit anti- TMPRSS2 (Abways, CY8435) and mouse anti- β- actin (HuaBio, HA601082). .. All computational studies were carried out using Discovery Studio (DS; Accelrys, San Diego, CA).

    Virus:

    Article Title: Sensing of cytoplasmic chromatin by cGAS activates innate immune response in SARS-CoV-2 infection
    Article Snippet: .. The antibodies used for immunoblotting were: rabbit-anti-cGAS (D1D3G, #15102 S, Cell Signaling Technology, 1:500), rabbit-anti-STING (D2P2F, #13647 S, Cell Signaling Technology, 1:500), rabbit-anti-phospho-STING (Ser366) (D7C3S, #19781 S, Cell Signaling Technology, 1:500), rabbit-anti-IRF3 (ab76409, Abcam, 1:1000), rabbi-anti-phospho-IRF3 (ab76493, Abcam, 1:1000), rabbit-anti-GAPDH (BE0024, EASYBIO, 1:2000), mouse-anti-β-Tubulin (CW0098, CWBIO, 1:2000), rabbit-anti-SARS-CoV-2 spike protein (40589-T62, Sino Biological, 1:1000), rabbit-anti-SARS-CoV-2 nucleocapsid protein (NP) (40143-R019, Sino Biological, 1:1000), rabbit-anti-Sendai virus (PD029C1, MBL, 1:1000), rabbit-anti-ACE2 (10108-T60, Sino Biological, 1:1000), mouse-anti-MAVS (sc166583, Santa Cruz, 1:500), goat-anti-mouse IgG-HRP secondary antibody (115035003, Jackson ImmunoResearch), mouse-anti-Lamin A/C (MABT538, Sigma-Aldrich, 1:500), rabbit-anti-Lamin B1 (ab16048, Abcam, 1:500), goat-anti-rabbit IgG-HRP secondary antibody (111035003, Jackson ImmunoResearch). ..



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